Degradation of Methylene Blue Dye by Cladosporium isolated from SP cane wash water

Abstract

Among others, the lignin is one of the components of sugarcane washing water for the production of sugar and alcohol. Face to its randomized nature, however, ligninolytic enzymes are able to degrade other recalcitrant compounds (also aromatic). This work aimed to evaluate the ability of fungus isolated from the lagoons of decantation of the effluent treatment station (ETS) of the “S.A. Usina Coruripe Açúcar e Álcool”, season 2004/05, in degradate methylene blue dye how indicator of ligninperoxidases (LiP)production. For that, a growth medium added of 0,01% of Methylene Blue (MB = 3,7-bis-dimethylamine-phenazothionium chloride) was used to evidence the production of LiP through the descolourization of this substratum, by the oxidation and, consequently demethylation of the dye. The decline of the absorbance of the filtered MB-cultures, the pH, conductivity and biomass (dry weight) were also evaluated. The MB showed itself to be a good marker for the production of LiP, allowing the screening of one fungus (Cladosporium sp.) that expressed that enzymatic activity in the studied effluent