Valorization of residues from beer production as a source of antioxidant bioactive compounds

Authors

  • Lilian Tatiani Dusman Tonin Universidade Tecnológica Federal do Paraná image/svg+xml
  • Ana Caroline Raimundini Aranha Universidade Tecnológica Federal do Paraná image/svg+xml
  • Danielli Andrea Nardino Universidade Tecnológica Federal do Paraná image/svg+xml
  • Virginia Sanches Coelho de Oliveira Trindade Universidade Tecnológica Federal do Paraná image/svg+xml
  • Isabella Salvat Lucas Universidade Tecnológica Federal do Paraná image/svg+xml
  • Naiara Leticia Gatz Universidade Tecnológica Federal do Paraná image/svg+xml

DOI:

https://doi.org/10.22481/exon.v12i1.20509

Keywords:

wort, extraction, drying, phenolic compouds, flavonoids, anthocyanins

Abstract

A large amount of solid waste from beer production is generated annually in Brazil, which could be  used as a potential source of bioactive compounds. The present work evaluated the hot trub of three  different types of beer, named according to the hops added: Bobek, Cascade and Hersbrucker. The  effect of different hydroalcoholic solvents on the extraction of total phenolics, flavonoids and  antioxidant activity by the DPPH, ABTS and chelating ability methods was evaluated. It was found  that the solvent had a significant effect on these factors. The levels of anthocyanins and yellow  flavonoids in the residues were quantified. For all these factors, the influence of the type of malt and  hops used in beer production was observed, which resulted in residues with different quantifications  of these compounds and different antioxidant potentials. By Pearson's correlation coefficient, it was  possible to observe a high and positive correlation between antioxidant activity by the three methods  and the levels of total phenolics or flavonoids. The three residues showed high chelating ability,  superior to the EDTA standard, which can be attributed to the presence of phenolic compounds and  humulones present in the hops. This research has shown that these brewery by-products are a good  source of antioxidant compounds, which could have potential applications in the food,  pharmaceutical and cosmetic industries.

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References

Scalbert, A.; Manach, C.; Morand, C.; Rémésy, C.; Jiménez, L. Dietary polyphenols and the prevention of diseases, Crit. Rev. Food Sci. Nutr., 45 (2005) 287–306.

Shahidi, F.; Zhong, Y. Novel antioxidants in food quality preservation and health promotion, Eur. J. Lipid Sci. Tech., 112 (2010) 930–940.

Halliwell B.; Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br. J. Pharmacol., 142 (2004 ) 231-55.

Schieber, A.; Stintzing, F.C.; Carle, R. By-products of plant food processing as a source of functional compounds — Recent developments, Trends Food Sci. Technol., 12 (2001) 401–413.

Patel, S. Hibiscus sabdariffa: An ideal yet under-exploited candidate for nutraceutical applications, Biomed. Prev. Nutr., 4 (2014) 23-27.

MAPA - Ministério da Agricultura, ANUÁRIO DA CERVEJA NO BRASIL 2018 — Ministério da Agricultura, Pecuária e Abastecimento, Governo do Brasil, 2018. Disponível em: http://www.agricultura.gov.br/assuntos/inspecao/produtos-vegetal/pasta-publicacoes DIPOV/anuario-da-cerveja-no-brasil-2018/view. Accesso em 30 Set 2019.

Duarte, I.F.; Barros, A.; Belton, P.S.; Righelato, R.; Spraul, M.; Humpfer, E.; Gil, A.M. High resolution NMR spectroscopy and multivariate analysis for the characterization of beer, J. Agr. Food Chem., 50 (2002) 2475–2481.

Siebert, K.J. Effects of protein-polyphenol interactions on beverage haze, stabilization, and analysis, J. Agr. Food Chem., 47 (1999) 353–362.

Dvorakova, M.; Moreira, M.M.; Dostalek, P.; Skulilova, Z.; Guido, L.F.; Barros, A.A. Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectroscopy, J. Chromatogr. A, 1189 (2008) 398-405.

Callemien, D.; Collin, S. Structure, organoleptic properties, quantification methods, and stability of phenolic compounds in beer – a review, Food Rev. Int, 26 (2010) 1–84.

Kerby, C.; Vriesekoop, F. An Overview of the Utilisation of Brewery By-Products as Generated by British Craft Breweries, Beverages, 3 (2017) 1-12.

Biele, B.C.; Marques, D.R.; Marchi, L.B.; Quelhas, J.O.F.; Chinellato, M.M.; Monteiro, C.C.F.; Monteiro, A.R.G. Produção de snack extrusado com adição de farinha de bagaço de malte, Rev. Tecnol., Edição Especial (2015) 321-326.

Silbir, S.; Goksungur, Y. Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain, Foods, 8 (2019) 1-14.

Keller-Reinspach, H.W. Emissions during the combustion of spent brewer’s grains, Brauwelt, 129 (1989) 2316–2319

Stocks, C.; Barker, A.J.; Guy, S. The composting of brewery sludge, J. Inst. Brew., 108 (2002) 452–458.

Sharp, F.R.; Laws, D.R.J. The essential oil of hops – a review, J. Inst. Brew., 87 (1981) 96-107. [17] Biendl, M. Hops and health, Tech. Quart., 46 (2009) 1–7.

Keukeleire, D.D.; Heyerick A.; Maes F. Production of hop extracts having oestrogenic and antiproliferative bioactivity, (2005) WO 2005058336 A1.

Pan, L.; Becker, H.; Gerhäuser, C. Xanthohumol induces apoptosis in cultured 40-16 human colon cancer cells by activation of the death receptorand mitochondrial pathway, Mol. Nutr. Food Res., 49 (2005) 837–843.

Proestos, C.; Komaitis, M. Antioxidant capacity of hops, em “Beer in Health and Disease Prevention” (Victor R. Preedy) Cap. 45, Elsevier Inc. Academic Press, 2009.

Bocquet L.; Sahpaz, S.; Hilbert, J. L.; Rambaud C.; Riviere, C. Humulus lupulus L., a very popular beer ingredientand medicinal plant: overview of its phytochemistry, its bioactivity, and its biotechnology, Phytochem. Rev., 17 (2018) 1047-1090.

Kammhuber, K. Differentiating between the world range of hop varieties according to bitter compounds and polyphenols, Hopfen Rundschau Int., 2005/2006 (2005) 42–46.

Pan, L.; BeckeR, H.; Gerhäuser, C. Xanthohumol induces apoptosis in cultured 40-16 human colon cancer cells by activation of the death receptorand mitochondrial pathway, Mol. Nutr. Food Res., 49 (2005) 837–843.

Sun, Z.; Zhou, C.; Liu, F.; Zhang, W.; Chen, J.; Pan, Y.; Ma, L.; Liu, Q.; Du, Y.; Yang, J.; Wang, Q. Inhibition of breast cancer cell survival by Xanthohumol via modulation of the Notch signaling pathway in vivo and in vitro, Oncol. Lett., 15 (2018) 908–916.

Festa, M.; Capasso, A.; D’Acunto, C.W.; Masullo, M.; Rossi, A.G.; Pizza, C.; Piacente, S. Xanthohumol induces apoptosis in human malignant glioblastoma cells by increasing reactive oxygen species and activating MAPK pathways, J. Nat. Prod., 74 (2011) 2505–2513.

Miranda, C.L.; Stevens, J.F.; Ivanov, V.; McCall, M.; Frei, B.; Deinzer, M.L.; Buhler, D.R. Antioxidant and prooxidant actions of prenylated and nonprenylated chalcones and flavanones in vitro, J. Agric. Food Chem., 48 (2000) 3876–3884.

Gerhauser, C. Broad spectrum anti-infective potential of xanthohumol from hop (Humulus lupulus L.) in comparison with activities of other hop constituents and xanthohumol metabolites, Mol. Nutr. Food Res., 49 (2005) 827–831.

Di Sotto, A.; Checconi, P.; Celestino, I.; Locatelli, M.; Carissimi, S.; De Angelis, M.; Rossi, V.; Limongi, D.; Toniolo, C.; Martinoli, L.; Di Giacomo, S.; Palamara, A.T.; Nencioni, L. Antiviral and Antioxidant Activity of a Hydroalcoholic Extract from Humulus lupulus L, Oxid. Med. Cell. Longev., 2018 (2018) 1-14.

Huige, N. Brewery by-products and effluents, em “Handbook of Brewing” (Priest, F.G., Stewart, G.G.) pp. 656–707, 2ª ed., Taylor & Francis Group: Boca Raton, FL, USA, 2006.

O’Rourke, T. Making the most of your hops, The New Brewer, 11 (1994) 20–33.

Bedini, S.; Flamini, G.; Girardi, J.; Cosci, F.; Conti, B. Not just for beer: Evaluation of spent hops (Humulus lupulus L.) as a source of eco-friendly repellents for insect pests of stored foods, J. Pest. Sci., 88 (2015) 583–592.

Huszcza, E.; Bartmanska, A.; Aniol, M.; Maczka, W.; Zolnierczyk, A.; Wawrzenczyk, C. Degradation of hop bitter acids by fungi, Waste Manage., 28 (2008) 1406-1410.

Fillaudeau, L.; Blanpain-Avet, P.; Daufin, G. Water, wastewater and waste management in brewing industries, J. Clean Prod., 14 (2006) 463–471.

Briggs, D.E.; Boulton, C.A.; Brookes, P.A., Stevens, R. “Brewing science and practice”, Cambridge, UK: Woodhead Publishing Limited and Boca Raton, FL, USA: CRC Press LLC, 2004.

Mathias, T.R.S; de Mello, P.P.M.; Sérvulo, E.F.C. Solid wastes in brewing process: a review, J. Brew. Distilling, 5 (2014) 1–9.

Cortese, M.; Gigliobianco, M.R.; Peregrina, D.V.; Sagratini, G.; Censi, R.; Di Martino, P. Quantification of phenolic compounds in different types of crafts beers, worts, starting and spent ingredients by liquid chromatography-tandem mass spectrometry, J. Chromatogr. A, 1612 (2020) 1- 11.

Francis, F.J. Analysis of anthocyanins, em “Anthocyanins as food colors” (Markakis, P.) pp. 181-207, New York: Academic Press, 1982.

Minussi, R.C; Rossi, M.; Bologna, L.; Cordi, L.; Rotilio, D.; Pastore, G.M.; Durán, N. Phenolic compounds and total antioxidant potential of commercial wines, Food Chem., 82 (2003) 409-416.

Funari, C.S.; Ferro, V.O. Análise de própolis, Ciên. Tecnol. Aliment., 26 (2006) 171–178.

Brand-Williams, W.; Cuvelier, M. E.; Berset, C. Use of free radical method to evaluate antioxidant activity, LWT-Food Sci. Technol., 22 (1995) 25-30.

Rufino, M.S.M; Alves, R.E.; Brito, E.S.; Morais, S.M.; Sampaio, C.G.; Jimenez, J.P.; Calixto, F.D.S. Determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH, Comunicado Técnico Embrapa, 127 (2007) 1-4.

Habeych, E.; Kogelenberg V.; Sagalowicz, L.; Michel, M.; Galaffu, N. Strategies to limit colour changes when fortifying food products with iron, Food Res. Int., 88 (2016) 122-128.

Celestino, S.M.C. Princípios de Secagem de Alimentos, Planaltina: Embrapa Cerrados, 2010.

Da Silva, C.F.G.; Suzuki, R.M.; Canesin, E.A.; Tonin; L.T.D. Otimização do processo de extração de compostos fenólicos do jiló (Solanum gilo Radi) e aplicação na estabilidade oxidativa do óleo de soja, Rev Virtual Quim., 9 (2017) 729-739.

Silva, Q.J.; Moreira, A.C.C.G.; Melo, E.A.; Lima, V.L.A.G. Compostos fenólicos e atividade antioxidante de genótipos de ciriguelas (Spondia Purpurea L.), Alimentos e Nutrição, 23 (2012) 73- 80.

Lapornik, B.; Prosek, M.; Wondra, A.G. Comparison of extract prepared from plant by products using different solvents and extraction time, J. Food Eng., 71 (2005) 214-222.

Kowalczyk, D.; Swieca, M.; Cichocka, J.; Gawlik-Dziki, U. The phenolic contente and antioxidante activity of the aqueous and hydroalcoholic extracts of hops and their pellets, J. I. Brewing, 119 (2013) 103-110.

Biendl, M. Research on the xanthohumol content in hops, Hopfen Rundschau Int., (2002) 72-75.

De Keukeleire, D.; De Cooman, L.; Rong, H.; Heyerick, A.; Kalita, J.; Milligan, S.R. “Functional properties of hop polyphenols. Plant Polyphenols 2: Chemistry, Biology, Pharmacology, Ecology”, Kluwer Academic, Plennum Publishers: New York, 1999.

Camargo, J.N.A.; Bertan, A.S.; Almeida, I.V.D.; Vicentini, V.E.P.; Dusman, E.; Tonin, L.T.D. Antitumoral activity, antioxidant capacity and bioactive compounds of ginger (Zingiber officinale), Acta Sci. Technol., 42 (2020) 1-11.

Santos, K.B.; Tonin, L.T.D. Estudo da influência da temperatura de secagem e solvente extrator na capacidade antioxidante de folhas Plantago major, Rev. Fitos, 13 (2019) 200-211.

Silva, C.F.G.; Mendes, M.P.; Almeida, V.V.; Michels, R.N.; Sakanaka, L.S.; Tonin, L.T.D. Parâmetros de qualidade físico-químicos e avaliação da atividade antioxidante de folhas de Plectranthus barbatus Andr. (Lamiaceae) submetidas a diferentes processos de secagem, Rev. Bras. Pl. Med., 18 (2016) 48-56.

Tonetti, C.R.; Suzuki, R.M.; Tonin, L.T.D. Efeito antioxidante do extrato do resíduo da produção do vinho na estabilidade oxidative do óleo de soja, Braz. J. Food Res., 7 (2016) 1-15.

Lopes, T.J.; Xavier, M.F.; Quadri, M.G.N.; Quadri, M.B. Antocianinas: Uma breve revisão das características estruturais e da estabilidade. Rev. Bras. Agroc., 13 (2007) 291-297.

Alves, C.Q.; David, J.M.; David, J.P.; Bahia, M.V.; Aguiar, R.M. Métodos para determinação de atividade antioxidante in vitro em substratos orgânicos. Quim. Nova, 33 (2010) 2202-2210.

Halliwell, B.; Gutteridge, J.M.C. “Free Radicals in Biology and Medicine”, 3aed, Oxford University Press: New York, 1999.

Karabín, M.; Hudcová, T.; Jelínek, L.; Dostálek, P. Biologically active compounds from hops and prospects for their use, Compr. Rev. Food Sci. Food Saf., 15 (2016) 542-567.

Tagashira, M.; Watanabe, M.; Uemitsu, N. Biosci. Antioxidative activity of hop bitter acids and their analogues, Biotechnol. Biochem., 59 (1995) 740-742.

Simpson, W.J.J. Cambridge prize lecture. Studies on the sensitivity of lactic acid bacteria to hop bitter acids, Inst. Brew., 99 (1993) 405-411.

Pateiro, M.; Bermúdez, R.; Lorenzo, J.M.; Franco, D. Effect of addition of natural antioxidants on the shelf-life of “chorizo”, a Spanish dry-cured sausage, Antioxidants, 4 (2015) 42-67.

Munekata, P.E.S.; Franco, D.; Trindade, M.A.; Lorenzo, J.M. Characterization of phenolic composition in chestnut leaves and beer residue by LC-DAD-ESI-MS. LWT– Food Sci. Technol., 68 (2016) 52-58.

Callegari-Jacques, S.M. “Bioestatística: Princípios e Aplicações”, São Paulo: Editora Artmed S.A., 2003.

Oskoueian, E.; Abdullah, N.; Hendra, R.; Karimi, E. Bioactive compounds, antioxidant, xanthine oxidase inhibitory, tyrosinase inhibitory and anti-inflammatory activities of selected agro-industrial by-products, Int. J. Mol. Sci, 12 (2011) 8610-8625.

Romero-Díez, R.; Rodríguez-Rojoa, S.; Cocero, M.J.; Duarte, C.M.M.; Matias, A.A.; Bronze, M.R. Phenolic characterization of aging wine lees: Correlation with antioxidant activities, Food Chem., 259 (2018) 188-195.

Published

2026-09-11

How to Cite

TONIN, Lilian Tatiani Dusman; ARANHA, Ana Caroline Raimundini; NARDINO, Danielli Andrea; TRINDADE, Virginia Sanches Coelho de Oliveira; LUCAS, Isabella Salvat; GATZ, Naiara Leticia. Valorization of residues from beer production as a source of antioxidant bioactive compounds. Exatas Online, [S. l.], v. 12, n. 1, p. 38–51, 2026. DOI: 10.22481/exon.v12i1.20509. Disponível em: https://periodicos2.uesb.br/exon/article/view/20509. Acesso em: 2 oct. 2026.