Quantificação da atividade de CMCase e FPase produzidas a partir da fermentação em estado sólido da palma forrageira

Tamires Carvalho dos Santos; Gleydison Amarante Soares; Thiago José Onorio Rocha; Alexsandra N. Ferreira; Clissiane Soares Viana Pacheco; Marcelo Franco

doi:10.22481/exon.v2i2.19435

Authors

Tamires Carvalho dos Santos Southwest Bahia State University
Gleydison Amarante Soares Southwest Bahia State University
Thiago José Onorio Rocha Southwest Bahia State University
Alexsandra N. Ferreira Southwest Bahia State University
Clissiane Soares Viana Pacheco Southwest Bahia State University
Marcelo Franco Southwest Bahia State University

DOI:

https://doi.org/10.22481/exon.v2i2.19435

Keywords:

Tempo de fermentação, Teor de água, Rhizopus sp

Abstract

The term solid state fermentation refers to the growth of microorganisms on solid matrix. The palm girl (Nopalea cochenillifera) grown in semi-arid region of Northeast can be an alternative culture medium and low economic cost. The objective of this study was to evaluate the effect of fermentation and water content on the kinetic activity of cellulases produced by fungal species Rhizopus sp. The palm being the only medium. The method used to quantify the enzyme used the filter paper and carboxymethylcellulose as a substrate. The unit ofenzyme activity (U/mL) was defined as the amount of enzyme capable of releasing 1 µmol of reducing sugars per minute at 50 °C, sugars quantified bythe DNS. The maximization of enzyme activity for CMCase (endoglucanase) was5.7 U/mL to 0.949 water activity at 96h, while for FPase (endoglucanse + exoglucanase) was. 8.7 U/mL to 0.993 activity water in 72h. The fermentation was conducted in a bacteriological incubator at 30 oC.The fungus synthesizedboth enzymes without the need.of any supplies or inducer addition of the cellulose presentin the waste and water.at different concentrations.

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